Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17862-40] to gamma Sarcoglycan
Suitable for: IP, IHC-P, WB
Reacts with: Human

Product name

Anti-gamma Sarcoglycan antibody [EPR17862-40]
See all gamma Sarcoglycan primary antibodies
Description

Rabbit monoclonal [EPR17862-40] to gamma Sarcoglycan
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skeletal muscle, MCF-7, Human fetal brain, Human fetal heart, Human fetal spleen or Human fetal kidney whole cell lysate. IHC-P: Human skeletal muscle.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17862-40
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weakly cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Exposure time: 15 seconds

Blocking/diluting buffer 5% NFDM/TBST

This protein is expressed in skeletal and heart muscle.
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µl

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Exposure time: 3 minutes

Blcking/Dilution buffer 5% NFDM/TBST

This protein is expressed in skeletal and heart muscle.
Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution

Lane 1 : Human fetal brain whole cell lysate
Lane 2 : Human fetal heart whole cell lysate
Lane 3 : Human fetal kidney whole cell lysate
Lane 4 : Human fetal spleen whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking/DIlution buffer 5% NFDM/TBST.
ab181602 Anti-GAPDH antibody [EPR16891] used as a control.
This protein is expressed in skeletal and heart muscle.
Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203112 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203112 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal kidney whole cell lysate10 µg (Input). Lane 2: ab203112 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203112 in Human fetal kidney whole cell lysate.
Exposure time: 3 seconds
Blocking/Dilution buffer 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human colon tissue is observed.

Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112)

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