Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Galectin 7 antibody [EPR4287] (ab108623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4287] to Galectin 7
  • Suitable for: WB, IP, IHC-P, ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Galectin 7 antibody [EPR4287]
    See all Galectin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR4287] to Galectin 7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HaCaT whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-Galectin 7 antibody [EPR4287] (ab108623)
    Flow Cytometry - Anti-Galectin 7 antibody [EPR4287] (ab108623)
    Flow Cytometry analysis of HaCaT (Human skin keratinocyte) cells labeling Galectin 7 with Purified ab108623 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-Galectin 7 antibody [EPR4287] (ab108623)
    Immunocytochemistry - Anti-Galectin 7 antibody [EPR4287] (ab108623)
    Immunocytochemistry analysis of HaCaT (Human skin keratinocyte) cells labeling Galectin 7 with Purified ab108623 at 1:1000 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] (ab108623)
    Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] (ab108623)

    Purified ab108623 at 1/50 dilution (2µg) immunoprecipitating Galectin 7 in HaCaT whole cell lysate.
    Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10µg
    Lane 2 (+): ab108623 + HaCaT whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108623 in HaCaT whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 15 kDa

  • Anti-Galectin 7 antibody [EPR4287] (ab108623)
    Anti-Galectin 7 antibody [EPR4287] (ab108623)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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