Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2774] to Galectin 3 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free
    See all Galectin 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2774] to Galectin 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247475 is the carrier-free version of ab76466 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247475 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    Western blot - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)

    This data was developed using ab76466, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: Galectin 3 (KO) knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76466 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76466 was shown to specifically react with Galectin 3 (KO) when Galectin 3 (KO) knockout samples were used. Wild-type and Galectin 3 (KO) knockout samples were subjected to SDS-PAGE. ab76466 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    Flow Cytometry - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    This data was developed using ab76466, the same antibody clone in a different buffer formulation.Overlay histogram showing THP1 cells stained with ab76466 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76466, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    Immunocytochemistry - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    This data was developed using ab76466, the same antibody clone in a different buffer formulation.ab76466, at a 1/100 dilution, staining Galectin 3 in HeLa cells by Immunofluorescence.
  • Western blot - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    Western blot - Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    All lanes : Anti-Galectin 3 antibody [EPR2774] (ab76466) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using ab76466, the same antibody clone in a different buffer formulation.

     

  • Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)
    Anti-Galectin 3 antibody [EPR2774] - BSA and Azide free (ab247475)

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