Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-Galectin 7 antibody [EPR19903] (ab206435)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19903] to Galectin 7
Suitable for: IHC-Fr, WB, IHC-P, IP, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Galectin 7 antibody [EPR19903]
See all Galectin 7 primary antibodies
Description

Rabbit monoclonal [EPR19903] to Galectin 7
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-Fr	Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat skin lysates; HaCaT whole cell lysate. IHC-P: Human skin and squamous carcinoma of the cervix tissues; mouse and rat skin tissues. IHC-Fr: Rat skin tissue. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells. IP: Human skin lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19903
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other

Western blot - Anti-Galectin 7 antibody [EPR19903] (ab206435)

All lanes : Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/2000 dilution

Lane 1 : Human skin lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Galectin 7 is specifically expressed in stratified squamous epithelium, but not in non-epithelial organs such as skeletal muscle. PMID: 9697310; PMID: 7729568.
Western blot - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Lanes 1-2 : Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/2000 dilution
Lane 3 : Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/1000 dilution

Lane 1 : Rat skin lysate
Lane 2 : Mouse skin lysate
Lane 3 : HaCaT (Human keratinocyte cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2: 30 seconds; Lane 3: 15 seconds.

Galectin 7 is specifically expressed in stratified squamous epithelium, but not in non-epithelial organs. PMID: 9697310; PMID: 7729568.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of human skin is observed (PMID: 23658821). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the cervix tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on tumor cells of squamous carcinoma of the cervix is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: No staining in human kidney (PMID: 9697310).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of mouse skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: No staining on mouse stomach (PMID: 9697310).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of rat skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skin tissue labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on epithelium of rat skin is observed. Nuclear counterstain: DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HACAT cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Flow cytometric analysis of 4% paraformaldehyde-fixed HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Galectin 7 was immunoprecipitated from 0.35 mg of human skin lysate with ab206435 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab206435 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human skin lysate 10µg (Input).

Lane 2: ab206435 IP in human skin lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206435 in human skin lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Galectin 7 antibody [EPR19903] (ab206435)

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