Anti-Flagellin antibody (ab93713)
Key features and details
- Rabbit polyclonal to Flagellin
- Suitable for: WB, ICC/IF
- Reacts with: Escherichia coli, Salmonella anatum, Salmonella selandia, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Flagellin antibody
See all Flagellin primary antibodies -
Description
Rabbit polyclonal to Flagellin -
Host species
Rabbit -
Specificity
This antibody is specific for bacterial Flagellin (FliC). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli, Salmonella anatum, Salmonella selandia, Recombinant fragment -
Immunogen
Purified recombinant Flagellin made in BL21 bacteria.
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Positive control
- Lysates transfected with purified flagellin, E. Coli total lysate or 293T transfected with recombinant protein.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Flagellin antibody (ab93713) at 1/10000 dilution
All lanes : 293T transfected with recombinant protein
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.
Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI. -
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.
Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.