Anti-FIP antibody (ab125184)
Key features and details
- Rabbit polyclonal to FIP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FIP antibody
See all FIP primary antibodies -
Description
Rabbit polyclonal to FIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human FIP aa 296-346.
-
Positive control
- Purchase matching WB positive control:Recombinant Human FIP protein
- HeLa, Jurkat and 293T whole cell lysates
-
General notes
Protein previously labeled as USF2.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-FIP antibody (ab125184)All lanes : Anti-FIP antibody (ab125184) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds
-
Immunoprecipitation - Anti-FIP antibody (ab125184)ab125184, at 6 µg/mg lysate, detecting FIP in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125184 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 10 seconds.
