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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper

Anti-FIP antibody (ab264330)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-FIP antibody (ab264330)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to FIP
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FIP antibody
    See all FIP primary antibodies
  • Description

    Rabbit polyclonal to FIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human FIP aa 1-50. The exact sequence is proprietary. NP_003358.1
    Database link: Q15853

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue; mouse renal cell carcinoma.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-FIP antibody (ab264330)
    Western blot - Anti-FIP antibody (ab264330)
    All lanes : Anti-FIP antibody (ab264330) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 37 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP antibody (ab264330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP antibody (ab264330)

    Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for FIP using ab264330 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

    Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

  • Immunoprecipitation - Anti-FIP antibody (ab264330)
    Immunoprecipitation - Anti-FIP antibody (ab264330)

    FIP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate  (1.0 mg per IP reaction; 20% of IP loaded) using ab264330 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264330 at 1 μg/ml.

    Lane 1: ab264330 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in Hela whole cell lysate.

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP antibody (ab264330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP antibody (ab264330)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for FIP using ab264330 at 1/1000 dilution in immunohistochemical analysis. DAB staining. Counter stained with hematoxylin.

    Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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