Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19241-46] to Fibronectin - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free
    See all Fibronectin primary antibodies

  • Description

    Rabbit monoclonal [EPR19241-46] to Fibronectin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse stomach tissue.

  • General notes

    Ab224689 is the carrier-free version of ab199056. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab224689 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fironectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of rat stomach (PMID: 650151).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa 1-6 (mouse hepatoma epithelial cell line) cells labeling Fibronectin with ab199056 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on Hepa1-6 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

  • Flow Cytometry - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
    Flow Cytometry - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling Fibronectin with ab199056 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

  • Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
    Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

    Fibronectin was immunoprecipitated from 0.35 mg of mouse plasma with ab199056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse plasma 10 µg (Input). 

    Lane 2: ab199056 IP in mouse plasma. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199056 in mouse plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Firbonectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of mouse stomach (PMID: 650151).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
    Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)

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