Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP24539-127] to Fibrinogen gamma chain
  • Suitable for: IHC-P, WB, IP, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Fibrinogen gamma chain antibody [EP24539-127]
    See all Fibrinogen gamma chain primary antibodies

  • Description

    Rabbit monoclonal [EP24539-127] to Fibrinogen gamma chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, ICCmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, Human plasma treated with PNGase F whole, Mouse plasma, Mouse plasma treated with PNGase F whole, Rat plasma, Rat liver, HepG2, Human liver, Mouse liver lysates. IHC-P: Human liver, Human pancreas, Human hepatocellular carcinoma tissues. ICC: HepG2 cells. IP: HepG2, Mouse plasma cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP24539-127

  • Isotype

    IgG

Images

  • Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    All lanes : Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924) at 1/5000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human plasma lysate treated with PNGase F whole cell lysate
    Lane 3 : Mouse plasma lysate
    Lane 4 : Mouse plasma lysate treated with PNGase F whole cell lysate
    Lane 5 : Rat plasma lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 50,100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21480334).

    Exposure time: Lanes 1-5: 10 seconds

    Lane 6: 37 seconds.

     

  • Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    All lanes : Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 3 : DU145 (human prostate carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 52 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: A549/DU145 (PMID: 17949478).

     Exposure time: 26 seconds.

     

  • Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    All lanes : Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924) at 1/1000 dilution

    Lane 1 : Human liver lysate
    Lane 2 : Mouse liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 50,100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: Lanes 1: 1 seconds.

    Lane 2: 26 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID:24648543). The section was incubated with ab281924 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in the blood plasma and stroma of human pancreas (PMID:16510577). The section was incubated with ab281924 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatocellular carcinoma (PMID:30863175). The section was incubated with ab281924 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunocytochemistry - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Fibrinogen gamma chain with ab281924 at 1/100 (5.38 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in HepG2 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)

    Fibrinogen gamma chain was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug.

    Lane 2: ab281924 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281924 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)

    Fibrinogen gamma chain was immunoprecipitated from 0.35 mg Mouse plasma lysate 10 ug with ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse plasma lysate 10 ug.

    Lane 2: ab281924 IP in Mouse plasma lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281924 in mouse plasma lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)
    Anti-Fibrinogen gamma chain antibody [EP24539-127A] (ab281924)

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