Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGFR2 antibody [EPR24679-79] (ab281925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24679-79] to FGFR2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FGFR2 antibody [EPR24679-79]
    See all FGFR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24679-79] to FGFR2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 and MCF7 whole cell lysate. IP: HAP1 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24679-79

  • Isotype

    IgG

Images

  • Western blot - Anti-FGFR2 antibody [EPR24679-79] (ab281925)
    Western blot - Anti-FGFR2 antibody [EPR24679-79] (ab281925)
    All lanes : Anti-FGFR2 antibody [EPR24679-79] (ab281925) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate
    Lane 2 : FGFR2 knockout HAP1 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 145/92 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Positive control: MCF-7 (PMID: 31217507).

    Exposure time: 3 mins.

  • Immunoprecipitation - Anti-FGFR2 antibody [EPR24679-79] (ab281925)
    Immunoprecipitation - Anti-FGFR2 antibody [EPR24679-79] (ab281925)

    FGFR2 was immunoprecipitated from 0.35 mg HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate 10 μg with ab281925 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab281925 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HAP1 whole cell lysate 10 µg.

    Lane 2: ab281925 IP in HAP1 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281925 in HAP1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

  • Anti-FGFR2 antibody [EPR24679-79] (ab281925)
    Anti-FGFR2 antibody [EPR24679-79] (ab281925)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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