Anti-Fetuin antibody (ab34505)
Key features and details
- Goat polyclonal to Fetuin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fetuin antibody -
Description
Goat polyclonal to Fetuin -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment (alpha 2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein (Human).
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Positive control
- WB: Human serum or purified Fetuin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Ab34505 was purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fetuin antibody (ab34505)All lanes : Anti-Fetuin antibody (ab34505) at 1/10000 dilution
Lane 1 : 250 ng of purified
human Fetuin
Lane 2 : 5µl of human serum at 1/50 dilution
Observed band size: 58 kDa why is the actual band size different from the predicted?
A doublet band or slightly lower molecular weight band (see lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.