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Cardiovascular Blood Serum Proteins

Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-fetal hemoglobin antibody [EPR9708(B)]
    See all fetal hemoglobin primary antibodies
  • Description

    Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin
  • Host species

    Rabbit
  • Specificity

    ab137096 recognises Hemoglobin gamma-1/2 chain.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human fetal hemoglobin. The exact sequence is proprietary.

  • Positive control

    • WB: Human red blood cell and K562 cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9708(B)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Other

Images

  • Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
    Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
    Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 16 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
    Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
    All lanes : Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Unpurified)

    Lane 1 : Human red blood cell
    Lane 2 : K562 (human lymphoblast from chronic myelogenous leukemia) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

    Predicted band size: 16 kDa



    Blocking and Diluting Buffer:5% NFDM/TBST

     

    Exposure time:3 min

  • Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
    Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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