Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-fetal hemoglobin antibody [EPR9708(B)]
See all fetal hemoglobin primary antibodies -
Description
Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin -
Host species
Rabbit -
Specificity
ab137096 recognises Hemoglobin gamma-1/2 chain. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human fetal hemoglobin. The exact sequence is proprietary.
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Positive control
- WB: Human red blood cell and K562 cell lysates.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9708(B) -
Isotype
IgG -
Research areas
Images
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Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
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All lanes : Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Unpurified)
Lane 1 : Human red blood cell
Lane 2 : K562 (human lymphoblast from chronic myelogenous leukemia) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 16 kDaBlocking and Diluting Buffer:5% NFDM/TBST
Exposure time:3 min
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