Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free
    See all Ferritin Heavy Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes only the Ferritin heavy chain.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247418 is the carrier-free version of ab75972 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247418 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    All lanes : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution

    Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from 1% SDS Hot lysis method.
    Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from RIPA lysis method
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 3 minutes


    This data was developed using ab75972, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)

    This data was developed using ab75972, the same antibody clone in a different buffer formulation.

    ab75972 staining Ferritin Heavy Chain in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
  • Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Lanes 1-3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution (unpurified)
    Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution (unpurified)
    Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution (unpurified)

    Lane 1 : Human liver lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
    Lanes 3-4 : Human brain lysate
    Lane 5 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab75972, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution + Human fetal liver at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 21 kDa



    This data was developed using ab75972, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa



    This data was developed using ab75972, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    This data was developed using ab75972, the same antibody clone in a different buffer formulation.Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in Human kidney tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    This data was developed using ab75972, the same antibody clone in a different buffer formulation.Unpurified ab75972 showing positive staining in normal liver tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    This data was developed using ab75972, the same antibody clone in a different buffer formulation.Unpurified ab75972 showing positive staining in normal breast tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    This data was developed using ab75972, the same antibody clone in a different buffer formulation.Unpurified ab75972 showing positive staining in normal tonsil tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
    Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)

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