Anti-FCRL2 antibody [EPR16122] (ab197897)
![Anti-FCRL2 antibody [EPR16122] (ab197897)](https://www.abcam.com/ps/products/197/ab197897/Images/ab197897-240394-ab197897A.jpg "Anti-FCRL2 antibody [EPR16122] (ab197897)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16122] to FCRL2
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-FCRL2 antibody [EPR16122]
See all FCRL2 primary antibodies -
Description
Rabbit monoclonal [EPR16122] to FCRL2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NAMALWA, HeLa, 293 cell lysates and Human tonsil tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16122 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FCRL2 antibody [EPR16122] (ab197897)Anti-FCRL2 antibody [EPR16122] (ab197897) at 1/1000 dilution + NAMALWA (human Burkitt's lymphoma ) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Exposure time: 30 seconds
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![Western blot - Anti-FCRL2 antibody [EPR16122] (ab197897)](https://www.abcam.com/ps/products/197/ab197897/Images/ab197897-240393-ab197897B.jpg)
Western blot - Anti-FCRL2 antibody [EPR16122] (ab197897)Anti-FCRL2 antibody [EPR16122] (ab197897) at 1/1000 dilution + Human tonsil lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Exposure time: 3 minutes
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![Western blot - Anti-FCRL2 antibody [EPR16122] (ab197897)](https://www.abcam.com/ps/products/197/ab197897/Images/ab197897-240392-ab197897C.jpg)
Western blot - Anti-FCRL2 antibody [EPR16122] (ab197897)Anti-FCRL2 antibody [EPR16122] (ab197897) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Exposure time: 30 seconds
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![Immunoprecipitation - Anti-FCRL2 antibody [EPR16122] (ab197897)](https://www.abcam.com/ps/products/197/ab197897/Images/ab197897-240544-ab197897-ip-amend.jpg)
Immunoprecipitation - Anti-FCRL2 antibody [EPR16122] (ab197897)Immunoprecipitation analysis of 293 (Human epithelial cells from embryonic kidney) whole cell extract labeling FCRL2 using ab197897 at 1/60 dilution (Lane 2).
Lane 3: IP using Rabbit monoclonal IgG (ab172730) ) instead of ab197897 in 293 (Human epithelial cells from embryonic kidney) whole cell extract.
Lane 1: Input: 10 μg 293 (Human epithelial cells from embryonic kidney) whole cell extract.
Subsequent WB detection was performed using ab197897 at 1/1000 dilution.
An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. -
![Anti-FCRL2 antibody [EPR16122] (ab197897)](https://www.abcam.com/ps/products/197/ab197897/Images/ab197897-5-benefits-of-recombinant-antibodies.png)
Anti-FCRL2 antibody [EPR16122] (ab197897)
