Pig Lipocalin-2 ELISA Kit (ab207924)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 4 pg/ml - 400 pg/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Pig Lipocalin-2 ELISA Kit
See all Lipocalin-2 / NGAL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% U1 8 220ng/ml = 2% U2 8 34.2ng/ml = 4% P1 8 134ng/ml = 2% P2 8 206ng/ml = 2% Inter-assay Sample n Mean SD CV% U1 8 220ng/ml = 4% U2 8 34.2ng/ml = 5% P1 8 134ng/ml = 3% P2 8 206ng/ml = 10% -
Sample type
Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 1 pg/ml -
Range
4 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine = 99 51.5pg/ml - 350pg/ml Plasma = 98 44.5pg/ml - 326pg/ml -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Abcam’s Pig Lipocalin-2 ELISA Kit (ab207924) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Neutrophil Gelatinase-Associated Lipocalin levels in urine, plasma or serum.
The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against pig NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.
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Notes
NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are small secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL’s ability to bind bacterial siderophores. As NGAL was first reported as oncogene 24p3 in the mouse, this name is still occasionally used to identify NGAL in other species.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 25x Wash Buffer Solution 1 x 40ml 5x Sample Diluent Buffer 1 x 50ml Biotinylated Pig-NGAL Antibody 1 x 12ml HRP-Streptavidin Solution 1 x 12ml Microwell plate, 96 precoated wells 1 x 96 tests Pig NGAL Standard (0 pg/mL) 1 x 1ml Pig NGAL Standard (10 pg/mL) 1 x 1ml Pig NGAL Standard (100 pg/mL) 1 x 1ml Pig NGAL Standard (20 pg/mL) 1 x 1ml Pig NGAL Standard (200 pg/mL) 1 x 1ml Pig NGAL Standard (40 pg/mL) 1 x 1ml Pig NGAL Standard (400 pg/mL) 1 x 1ml Stop Solution 1 x 12ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Alternative names
- 24p3
- 25 kDa alpha 2 microglobulin related subunit of MMP9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
see all
Images
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Abcam’s Pig Lipocalin-2 ELISA Kit (ab207924) Calibration Curve
Abcam’s Pig Lipocalin-2 ELISA Kit (ab207924) Calibration Curve