Ab68889 staining human GCET1 in Burkitt's Lymphoma (A), Follicular B Cell Lymphoma (B), Diffuse Large B Cell Lymphoma (C) and Lymphocyte Predominant Hodgkin's Lymphoma (D) by immunohistochemistry using formalin fixed, paraffin embedded tissue.

IHC image of GCET1 staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68889, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

 

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Western blot results of GCET1 from transfected cell lines. The first two lanes demonstrate the specificity of ab68889 (1:100) on lysates of transfected HEK-GCET1 cell lines. A 46kDa band specific to the GCET1 protein was observed in GC-DLBCL (SUDHL4 and SUDHL6) and some other DLBCL (KARPAS 422, DB and HT). The GCET1 protein was not present in a subset of DLBCL (MHHPREB1, DOHH2).

The top bands show the GCET1 protein, whereas the bottom bands are GAPDH, used as a reference.

Ab68889 staining human GCET1 in human tonsil by immunohistochemistry using paraffin embedded tissue.