Anti-FBXO40 antibody [EPR15005] (ab190688)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15005] to FBXO40
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-FBXO40 antibody [EPR15005]
See all FBXO40 primary antibodies -
Description
Rabbit monoclonal [EPR15005] to FBXO40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart tissue lysate. Human skeletal muscle tissue lysate. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15005 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FBXO40 antibody [EPR15005] (ab190688) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
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Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling FBXO40 with ab190688 at 1/90 followed by Goat anti rabbit IgG (FITC) at 1/150 (red). Rabbit monoclonal IgG was used as isotype control (green).
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Anti-FBXO40 antibody [EPR15005] (ab190688) at 1/10000 dilution + Human fetal heart lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
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