Anti-Cullin 4B/CUL-4B antibody (ab227724)
Key features and details
- Rabbit polyclonal to Cullin 4B/CUL-4B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cullin 4B/CUL-4B antibody
See all Cullin 4B/CUL-4B primary antibodies -
Description
Rabbit polyclonal to Cullin 4B/CUL-4B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Cullin 4B/CUL-4B (internal sequence). The exact sequence is proprietary.
Database link: Q13620 -
Positive control
- WB: Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2, HEK-293T, A431, HeLa, HepG2 and A-375 whole cell lysate. IHC: Human esophagus carcinoma tissue. ICC/IF: HeLa cells.
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General notes
Protein previously labeled as Cullin 4B.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
Lanes 1 & 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8D30 whole cell lysate
Lane 3 : NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa5% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cullin 4B/CUL-4B (green) using ab227724 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red - phalloidin cytoskeletal marker.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4B/CUL-4B antibody (ab227724)
Paraffin-embedded human esophagus carcinoma tissue stained for Cullin 4B/CUL-4B using ab227724 at 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa5% SDS-PAGE
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All lanes : Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa5% SDS-PAGE
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Anti-Cullin 4B/CUL-4B antibody (ab227724) at 1/1000 dilution + A-375 (human malignant melanoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa5% SDS-PAGE