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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes SCF Complex E3 Ligase

Anti-Cullin 4B/CUL-4B antibody (ab67035)

Price and availability

308 236 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Cullin 4B/CUL-4B antibody (ab67035)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse polyclonal to Cullin 4B/CUL-4B
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cullin 4B/CUL-4B antibody
    See all Cullin 4B/CUL-4B primary antibodies
  • Description

    Mouse polyclonal to Cullin 4B/CUL-4B
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length protein corresponding to Human Cullin 4B/CUL-4B. (NP_003579, 1 a.a. ~ 913 a.a)

  • Positive control

    • WB: Transfected 293T cell lysate ICC/IF: HeLa cells
  • General notes

    Protein previously labeled as Cullin 4B.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase

Images

  • Western blot - Anti-Cullin 4B/CUL-4B antibody (ab67035)
    Western blot - Anti-Cullin 4B/CUL-4B antibody (ab67035)
    All lanes : Anti-Cullin 4B/CUL-4B antibody (ab67035) at 1/500 dilution

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : non Transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size: 103 kDa
    Observed band size: 99 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (ab67035)
    Immunocytochemistry/ Immunofluorescence - Anti-Cullin 4B/CUL-4B antibody (ab67035)

    ab67035, at a concentration 10 µg/ml, staining Cullin 4B/CUL-4B in HeLa cell by Immunofluorescence.

  • Western blot - Anti-Cullin 4B/CUL-4B antibody (ab67035)
    Western blot - Anti-Cullin 4B/CUL-4B antibody (ab67035) This image is courtesy of an Abreview submitted by Svetlana Khoronenkova
    All lanes : Anti-Cullin 4B/CUL-4B antibody (ab67035) at 1/500 dilution (Blocker 1:1 in PBS + 0.1% Tween for 14 hours at 4°C)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Cul4 siRNA in HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : An Alexa Fluor®680-conjugated Goat anti-mouse polyclonal at 1/10000 dilution

    Predicted band size: 103 kDa



    Blocking Step:LI-COR® Odyssey®Blocking Buffer (50%) for 45 minutes at room temperature.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Cullin 4B/CUL-4B antibody (ab67035)

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    Applications: ICC/IF, IHC-P, WB

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    Applications: WB

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    Anti-Cullin 4B/CUL-4B antibody (ab157103)

    Applications: WB

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