Anti-Cullin 4B/CUL-4B antibody (ab67035)
Key features and details
- Mouse polyclonal to Cullin 4B/CUL-4B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cullin 4B/CUL-4B antibody
See all Cullin 4B/CUL-4B primary antibodies -
Description
Mouse polyclonal to Cullin 4B/CUL-4B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Full length protein corresponding to Human Cullin 4B/CUL-4B. (NP_003579, 1 a.a. ~ 913 a.a)
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Positive control
- WB: Transfected 293T cell lysate ICC/IF: HeLa cells
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General notes
Protein previously labeled as Cullin 4B.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cullin 4B/CUL-4B antibody (ab67035) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : non Transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 103 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
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ab67035, at a concentration 10 µg/ml, staining Cullin 4B/CUL-4B in HeLa cell by Immunofluorescence.
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All lanes : Anti-Cullin 4B/CUL-4B antibody (ab67035) at 1/500 dilution (Blocker 1:1 in PBS + 0.1% Tween for 14 hours at 4°C)
Lane 1 : HeLa whole cell lysate
Lane 2 : Cul4 siRNA in HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : An Alexa Fluor®680-conjugated Goat anti-mouse polyclonal at 1/10000 dilution
Predicted band size: 103 kDa
Blocking Step:LI-COR® Odyssey®Blocking Buffer (50%) for 45 minutes at room temperature.