Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)
![Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)](https://www.abcam.com/ps/products/276/ab276073/Images/ab276073-2-anti-dcun1d1-antibody-ip-hela-a549-hut78-cells.jpg "Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [001] to DCUN1D2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-DCUN1D2 antibody [001] - BSA and Azide free -
Description
Rabbit monoclonal [001] to DCUN1D2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human DCUN1D2 aa 1-259.
Database link: Q6PH85 -
Positive control
- WB: HCT 116 whole cell lysate. IP: HeLa, A549, HuT 78 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
001 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-DCUN1D2 antibody [001] (ab276073)
DCUN1D2 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) (Lane A), A549 (Human lung carcinoma cell line) (Lane B) and HuT 78 (human cutaneous T lymphocyte Sezary Syndrome cell line) whole cell lysates with 2 µL ab276073 and 15 µl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitates using ab276073 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions. -
![Western blot - Anti-DCUN1D2 antibody [001] (ab276073)](https://www.abcam.com/ps/products/276/ab276073/Images/ab276073-1-anti-dcun1d1-antibody-wb-hct116-cells.jpg)
Western blot - Anti-DCUN1D2 antibody [001] (ab276073)Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073) at 1/500 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 30 kDaDeveloped using the Odyssey technique.
Performed under reducing conditions. -
![Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)](https://www.abcam.com/ps/products/276/ab276073/Images/ab276073-3-benefits-of-recombinant-antibodies.png)
Anti-DCUN1D2 antibody [001] - BSA and Azide free (ab276073)