Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3372] to Factor XIIIa - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free
    See all Factor XIIIa primary antibodies

  • Description

    Rabbit monoclonal [EP3372] to Factor XIIIa - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-Fr: mouse colon, and mouse spleen tissues IHC-P: mouse testis, mouse placenta and human skin tissues.

  • General notes

    Ab232426 is the carrier-free version of ab76105. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232426 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)
    Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

    Immunohistochemistry (Frozn sections) analysis of mouse colon tissue sections labeling Factor XIIIa with purified ab76105 at 1:50 (1.3µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76105)

  • Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)
    Immunohistochemistry (Frozen sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

    Immunohistochemistry (Frozn sections) analysis of mouse spleen tissue sections labeling Factor XIIIa with purified ab76105 at 1:50 (1.3µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76105)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Factor XIIIa with purified ab76105 at 1:1000 (0.27 µg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76105).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse placenta tissue sections labeling Factor XIIIa with purified ab76105 at 1:1000 (0.27 µg/ml). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76105)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

    ab76105, at 1/100 dilution, staining Factor XIIIa in paraffin-embedded human skin tissue by Immunohistochemistry. Note positive staining of dendrocytes within the papillary dermis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76105).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)
    Anti-Factor XIIIa antibody [EP3372] - BSA and Azide free (ab232426)

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