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Anti-Factor XII antibody [EPR22292-127] (ab242123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22292-127] to Factor XII
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor XII antibody [EPR22292-127]
    See all Factor XII primary antibodies

  • Description

    Rabbit monoclonal [EPR22292-127] to Factor XII

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Natural Human Factor XII protein; Human plasma and serum. IP: Human plasma and serum.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22292-127

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123)
    Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123)
    Anti-Factor XII antibody [EPR22292-127] (ab242123) at 1/1000 dilution + Native Human Factor XII protein (ab62423) at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 50,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Factor XII is an 80-kDa polypeptide undergoes catalysis and forms the 50-kDa heavy and 30-kDa light chains of alpha-factor XIIa. The molecular weight observed is consistent with literature (PMID: 28069606; 2473984; 8049433).
  • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (ab242123)
    Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (ab242123)

    Factor XII was immunoprecipitated from 0.35 mg of human plasma with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human plasma 10 µg (Input). 

    Lane 2: ab242123 IP in human plasma. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242123 in human plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID: 8049433, PMID 2473984).

  • Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123)
    Western blot - Anti-Factor XII antibody [EPR22292-127] (ab242123)
    All lanes : Anti-Factor XII antibody [EPR22292-127] (ab242123) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human serum

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 50,80 kDa why is the actual band size different from the predicted?



    Exposure time : Lane 1:8 Seconds; Lane 2:3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Factor XII is an 80-kDa polypeptide undergoes catalysis and forms the 50-kDa heavy and 30-kDa light chains of alpha-factor XIIa. The molecular weight observed is consistent with literature (PMID: 28069606; 2473984; 8049433).

  • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (ab242123)
    Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] (ab242123)

    Factor XII was immunoprecipitated from 0.35 mg of human serum with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human serum 10 µg (Input). 

    Lane 2: ab242123 IP in human serum. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242123 in human serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID: 8049433, PMID 2473984).

  • Anti-Factor XII antibody [EPR22292-127] (ab242123)
    Anti-Factor XII antibody [EPR22292-127] (ab242123)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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