Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor X antibody [EPR16249] (ab196023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16249] to Factor X
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor X antibody [EPR16249]
    See all Factor X primary antibodies

  • Description

    Rabbit monoclonal [EPR16249] to Factor X

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, Human blood, Human plasma and HepG2 lysates; Human liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16249

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Factor X antibody [EPR16249] (ab196023)
    Western blot - Anti-Factor X antibody [EPR16249] (ab196023)
    All lanes : Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human blood lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    We hypothesis the 74 kDa band is the precursor of Factor X.
    Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor X antibody [EPR16249] (ab196023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor X antibody [EPR16249] (ab196023)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Factor X with ab196023 at 1/1200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Factor X antibody [EPR16249] (ab196023)
    Western blot - Anti-Factor X antibody [EPR16249] (ab196023)
    Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + Human plasma lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    We hypothesis the 74 kDa band is the precursor of Factor X.
    Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479.

  • Western blot - Anti-Factor X antibody [EPR16249] (ab196023)
    Western blot - Anti-Factor X antibody [EPR16249] (ab196023)
    Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    We hypothesis the 74 kDa band is the precursor of Factor X.
    Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479.

  • Anti-Factor X antibody [EPR16249] (ab196023)
    Anti-Factor X antibody [EPR16249] (ab196023)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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