Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22292-127] to Factor XII - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free
    See all Factor XII primary antibodies

  • Description

    Rabbit monoclonal [EPR22292-127] to Factor XII - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human plasma and serum.

  • General notes

    Ab242152 is the carrier-free version of ab242123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22292-127

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
    Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

    Factor XII was immunoprecipitated from 0.35 mg of human serum with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human serum 10 µg (Input). 

    Lane 2: ab242123 IP in human serum. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242123 in human serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID: 8049433, PMID 2473984).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242123).

  • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
    Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

    Factor XII was immunoprecipitated from 0.35 mg of human plasma with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human plasma 10 µg (Input). 

    Lane 2: ab242123 IP in human plasma. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242123 in human plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID: 8049433, PMID 2473984).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242123).

  • Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
    Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

© 2021 Astana Biomed Group LLP. All rights reserved.