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Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14840(B)] to EXOSC3 - N-terminal
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal
    See all EXOSC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14840(B)] to EXOSC3 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa, Jurkat and Raji cell lysates; Human colon tissue; Human transitional cell carcinoma of bladder tissue; 293T and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14840(B)

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling EXOSC3 with ab184961 at 1/100 dilution (red). Counterstained with Dapi (blue). 

  • Flow Cytometry - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Flow Cytometry - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling EXOSC3 with ab184961 at 1/100 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    All lanes : Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961) at 1/10000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : Raji cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunoprecipitation analysis of 293T lysates (Lane 1) labeling EXOSC3 with ab184961 at a 1/50 dilution and a negative control PBS (Lane 2).  Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EXOSC3 with ab184961 at 1/50 dilution.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human colon tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
    Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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