Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14841] to EXOSC3 - C-terminal
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EXOSC3 antibody [EPR14841] - C-terminal
    See all EXOSC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14841] to EXOSC3 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • RAW264.7, 293T, Jurkat, HeLa, NIH 3T3, mouse kidney tissue and mouse spleen tissue lysate. Rat kidney tissue lysate. Rat spleen tissue lysate. Human ovarian carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14841

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : RAW264.7 cell line lysate
    Lane 6 : NIH 3T3 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

    Immunofluorescent analysis of 4% paraformaldehyde fixed RAW264.7 cells labeling EXOSC3 with ab190689 at 1/250, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and counterstained with DAPI.

  • Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

    Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.

    Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.



    All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

    Lane 1 : Lysate of 293T cells immunoprecipated with ab190689 at 1/50
    Lane 2 : PBS

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

    Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

    Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

    Lane 1 : Jurkat cell line lysate
    Lane 2 : HeLa cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + 293T cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)
    Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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