Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14841] to EXOSC3 - C-terminal
Suitable for: ICC/IF, IP, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-EXOSC3 antibody [EPR14841] - C-terminal
See all EXOSC3 primary antibodies
Description

Rabbit monoclonal [EPR14841] to EXOSC3 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- RAW264.7, 293T, Jurkat, HeLa, NIH 3T3, mouse kidney tissue and mouse spleen tissue lysate. Rat kidney tissue lysate. Rat spleen tissue lysate. Human ovarian carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14841
Isotype

IgG
Research areas

Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : RAW264.7 cell line lysate
Lane 6 : NIH 3T3 cell line lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Immunofluorescent analysis of 4% paraformaldehyde fixed RAW264.7 cells labeling EXOSC3 with ab190689 at 1/250, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and counterstained with DAPI.
Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.

All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

Lane 1 : Lysate of 293T cells immunoprecipated with ab190689 at 1/50
Lane 2 : PBS

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Observed band size: 30 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution

Lane 1 : Jurkat cell line lysate
Lane 2 : HeLa cell line lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + 293T cell line lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689)

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