Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERK1 antibody [12D11] (ab119357)

Key features and details

  • Mouse monoclonal [12D11] to ERK1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human, Non human primates, African green monkey
  • Isotype: IgG

Overview

  • Product name

    Anti-ERK1 antibody [12D11]
    See all ERK1 primary antibodies

  • Description

    Mouse monoclonal [12D11] to ERK1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant full length Human ERK1 expressed in bacteria.

  • Positive control

    • 293, U2OS, K562, A549, HeLa, Jurkat, MCF7, HepG2, MEF, NIH 3T3, C2C12 and COS7 cell lysates; HeLa cells; Human colon cancer tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ERK1 antibody [12D11] (ab119357)
    Western blot - Anti-ERK1 antibody [12D11] (ab119357)
    All lanes : Anti-ERK1 antibody [12D11] (ab119357) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : U2OS cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : HeLa cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MCF7 cell lysate
    Lane 8 : HepG2 cell lysate
    Lane 9 : MEF cell lysate
    Lane 10 : NIH 3T3 cell lysate
    Lane 11 : C2C12 cell lysate
    Lane 12 : COS7 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse-HRP at 1/25000 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    4-20% Tris-HCl polyacrylamide gel.
  • Immunocytochemistry/ Immunofluorescence - Anti-ERK1 antibody [12D11] (ab119357)
    Immunocytochemistry/ Immunofluorescence - Anti-ERK1 antibody [12D11] (ab119357)
    ab119357, at 1/100 dilution, staining ERK1 in untreated and siRNA knockdown treated HeLa cells (Formalin fixed and permeabilized with 0.1% Triton X-100) by Immunoflourescence followed by DyLight 549 goat-anti-mouse secondary antibody at a dilution of 1/400.
    Nuclei (blue) were stained with Hoechst 33342 dye.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 antibody [12D11] (ab119357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 antibody [12D11] (ab119357)
    ab119357, at 1/100 dilution, staining ERK1 in deparaffinized Human colon cancer tissue by Immunohistochemistry using a goat anti-mouse HRP secondary antibody at a dilution of 1/500 and colorimetric detection using metal enhanced DAB.
    Note: strong cytoplasmic localization of ERK1.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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