HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)
![HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)](https://www.abcam.com/ps/products/237/ab237256/Images/ab237256-344422-237256WB1jpg.jpg "HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
See all Ubiquitin primary antibodies -
Description
HRP Rabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27) -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Di-Ubiquitin/Ub2 (K27 Linked).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17034 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)
This blot was produced using a 4-12% Bis-Tris gel under the MES buffer system. The gel was run at 200V for 35 mins. before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab237256 (1/1000) overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Samples:
Lane 1. Di-Ubiquitin/Ub2 (Full Length) (20ng)
Lane 2. Di-Ubiquitin/Ub2 (K11 Linked) (20ng)
Lane 3. Di-Ubiquitin/Ub2 (K27 Linked) (20ng)
Lane 4. Di-Ubiquitin/Ub2 (K29 Linked) (20ng)
Lane 5. Di-Ubiquitin/Ub2 (K33 Linked ) (20ng)
Lane 6. Di-Ubiquitin/Ub2 (K48 Linked) (20ng)
Lane 7. Di-Ubiquitin/Ub2 (K6 Linked) (20ng)
Lane 8. Di-Ubiquitin/Ub2 (K63 Linked) (20ng)
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![HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)](https://www.abcam.com/ps/products/237/ab237256/Images/ab237256-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab237256)