Anti-eRF1 antibody (ab31799)
Key features and details
- Rabbit polyclonal to eRF1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-eRF1 antibody
See all eRF1 primary antibodies -
Description
Rabbit polyclonal to eRF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human eRF1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab31798)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab31799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. -
Sequence similarities
Belongs to the eukaryotic release factor 1 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 541077 Cow
- Entrez Gene: 2107 Human
- Entrez Gene: 225363 Mouse
- Entrez Gene: 100009553 Rabbit
- Entrez Gene: 307503 Rat
- Entrez Gene: 399462 Xenopus laevis
- Omim: 600285 Human
- SwissProt: P62495 Human
see all -
Alternative names
- Cl1 protein antibody
- D5S1995 antibody
- ERF antibody
see all
Images
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Western blot - Anti-eRF1 antibody (ab31799)All lanes : Anti-eRF1 antibody (ab31799) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Western blot - Anti-eRF1 antibody (ab31799)All lanes : Anti-eRF1 antibody (ab31799) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Liver (Rat) Tissue Lysate
Lane 8 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (ab31799)ICC/IF image of ab31799 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31799, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
References (2)
ab31799 has been referenced in 2 publications.
- Huang L et al. Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations. Nucleic Acid Ther 29:175-186 (2019). PubMed: 31070517
- Liu P et al. Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality. Mol Cell Biol 30:4245-53 (2010). WB, IP ; Human . PubMed: 20606008
Images
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Western blot - Anti-eRF1 antibody (ab31799)All lanes : Anti-eRF1 antibody (ab31799) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Western blot - Anti-eRF1 antibody (ab31799)All lanes : Anti-eRF1 antibody (ab31799) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Liver (Rat) Tissue Lysate
Lane 8 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (ab31799)ICC/IF image of ab31799 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31799, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
