Anti-Ephrin B3 antibody (ab101699)
Key features and details
- Rabbit polyclonal to Ephrin B3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Ephrin B3 antibody
See all Ephrin B3 primary antibodies -
Description
Rabbit polyclonal to Ephrin B3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Cat, Dog, Pig, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human Ephrin B3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab113310) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 36 kDa). Target
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Function
May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. -
Tissue specificity
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments. -
Sequence similarities
Belongs to the ephrin family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1949 Human
- Entrez Gene: 13643 Mouse
- Entrez Gene: 360546 Rat
- Omim: 602297 Human
- SwissProt: Q15768 Human
- SwissProt: O35393 Mouse
- Unigene: 26988 Human
- Unigene: 249637 Mouse
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Alternative names
- EFL 6 antibody
- EFL6 antibody
- EFNB 3 antibody
see all
Images
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Western blot - Anti-Ephrin B3 antibody (ab101699)All lanes : Anti-Ephrin B3 antibody (ab101699) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lane 5 : Rat Forebrain
Lane 6 : E10 Mouse Embryo Brain Tissue Lysate
Lane 7 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
Ephrin B3 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B3 antibody (ab101699)ICC/IF image of ab101699 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab101699 has been referenced in 3 publications.
- Xiong Y et al. Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury. Mol Med Rep 18:2171-2181 (2018). PubMed: 29901151
- Efazat G et al. Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer. Oncotarget 7:60332-60347 (2016). PubMed: 27533087
- Qu B et al. Testing stem cell therapy in a rat model of inflammatory bowel disease: role of bone marrow stem cells and stem cell factor in mucosal regeneration. PLoS One 9:e107891 (2014). IHC ; Rat . PubMed: 25309991
Images
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Western blot - Anti-Ephrin B3 antibody (ab101699)All lanes : Anti-Ephrin B3 antibody (ab101699) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lane 5 : Rat Forebrain
Lane 6 : E10 Mouse Embryo Brain Tissue Lysate
Lane 7 : E14 Mouse Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
Ephrin B3 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B3 antibody (ab101699)ICC/IF image of ab101699 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101699, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
