Anti-Eph receptor B4/HTK antibody [04] - BSA and Azide free (ab277130)
![Anti-Eph receptor B4/HTK antibody [04] - BSA and Azide free (ab277130)](https://www.abcam.com/ps/products/277/ab277130/Images/ab277130-2-AntiEph-receptor-B4HTK-antibody-ICC-MCF7-cells.jpg "Anti-Eph receptor B4/HTK antibody [04] - BSA and Azide free (ab277130)")
Key features and details
- Mouse monoclonal [04] to Eph receptor B4/HTK - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Eph receptor B4/HTK antibody [04] - BSA and Azide free
See all Eph receptor B4/HTK primary antibodies -
Description
Mouse monoclonal [04] to Eph receptor B4/HTK - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
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Immunogen
Recombinant fragment corresponding to Human Eph receptor B4/HTK aa 1-539. Genbank: NP_004435.3
Database link: P54760 -
Positive control
- Flow cyt: MCF-7 cells ICC: MCF-7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
04 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Eph receptor B4/HTK antibody (ab277130)
Immunofluorescence staining of EPhB4 in MCF-7 (human breast adenocarcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277130 at 1/30 dilution at 4° overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI (blue).
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Flow Cytometry - Anti-Eph receptor B4/HTK antibody (ab277130)Flow cytometric analysis of EphB4 expression on MCF-7 (human breast adenocarcinoma cell line) cells. Cells were stained with ab277130 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also stained with an isotype control (Grey). The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
