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Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8280] to Eph receptor B3 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free
    See all Eph receptor B3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8280] to Eph receptor B3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HepG2, HeLa, MOLT4, NIH3T3, Rat-1 and C6 cell lysates. Rat spinal cord tissue lysate; ICC/IF: Jurkat and HeLa cells.

  • General notes

    Ab248634 is the carrier-free version of ab133742. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248634 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)
    Western blot - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)
    All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 7 : Rat spinal cord lysates
    Lane 8 : Rat-1 (Rat embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133742).

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)
    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)
    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133742).
  • Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)
    Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)

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