Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free (ab251087)
![Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free (ab251087)](https://www.abcam.com/ps/products/251/ab251087/Images/ab251087-399084-anti-eph-receptor-a3-phospho-y596-antibody-epr3434-n-bsa-and-azide-free-western-blot.jpg "Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free (ab251087)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3434(N)] to Eph receptor A3 (phospho Y596) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free
See all Eph receptor A3 primary antibodies -
Description
Rabbit monoclonal [EPR3434(N)] to Eph receptor A3 (phospho Y596) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251087 is the carrier-free version of ab191565. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251087 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR3434(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free (ab251087)All lanes : Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] (ab191565) at 1/10000 dilution
Lane 1 : Lysate from 293 cells treated with pervanadate
Lane 2 : Lysate from untreated 293 cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?This data was developed using ab191565, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free (ab251087)](https://www.abcam.com/ps/products/251/ab251087/Images/ab251087-2-benefits-of-recombinant-antibodies.png)
Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] - BSA and Azide free (ab251087)
