Anti-ELKS antibody [EPR13777] (ab180507)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13777] to ELKS
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-ELKS antibody [EPR13777]
See all ELKS primary antibodies -
Description
Rabbit monoclonal [EPR13777] to ELKS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, HEK293, Jurkat and SW480 cell lysate. SW480 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13777 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-ELKS antibody [EPR13777] (ab180507) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 128 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
-
Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).
-