Anti-eIF3l antibody - N-terminal (ab230521)
Key features and details
- Rabbit polyclonal to eIF3l - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF3l antibody - N-terminal
See all eIF3l primary antibodies -
Description
Rabbit polyclonal to eIF3l - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog, Pig, Monkey
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Immunogen
Synthetic peptide corresponding to Human eIF3l (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9Y262 -
Positive control
- IHC-P: Human placenta tissue. ICC/IF: HEK-293 cells. WB: HEK-293 and A431 whole cell lysate.
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General notes
This product was previously labelled as IF3EI
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF3l antibody - N-terminal (ab230521)All lanes : Anti-eIF3l antibody - N-terminal (ab230521) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 66 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-eIF3l antibody - N-terminal (ab230521)HEK-293 (Human epithelial cell line from embryonic kidney) cells stained for eIF3l using ab230521 at a dilution of 1/100 in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab230521 in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3l antibody - N-terminal (ab230521)Formalin-fixed, paraffin-embedded human placenta tissue stained for eIF3l with ab230521 at a 1:100 dilution in immunohistochemical analysis.
