Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23228-40] to EGR2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free
    See all EGR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23228-40] to EGR2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ChIP,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse P0 cerebellum and Mouse thymus tissue lysates; Rat thymus and Rat brain lysates. IHC-P: Mouse cerebrum tissue; Rat cerebrum and Rat skin tissue. Flow Cyt: Mouse splenocytes treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h.

  • General notes

    ab270755 is the carrier-free version of ab254228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat skin (PMID: 21514423, 21514423).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

  • Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
    Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

    Flow cytometric analysis of 2% paraformaldehyde fixed, True-Nuclear buffer-permeabilized Mouse splenocyte (treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h) cells labelling EGR2 with ab245228 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD4 conjugated to APC-Cy7 . Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG (Left) or ab245228 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat cerebrum.The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the mouse cerebrum (PMID: 30122531).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245228).

  • Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)
    Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free (ab270755)

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