Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGR2 antibody [EPR23228-40] (ab245228)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23228-40] to EGR2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-EGR2 antibody [EPR23228-40]
    See all EGR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23228-40] to EGR2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse P0 cerebellum and Mouse thymus tissue lysates; Rat thymus and Rat brain lysates. IHC-P: Mouse cerebrum tissue; Rat cerebrum and Rat skin tissue. Flow Cyt: Mouse splenocytes treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    All lanes : Anti-EGR2 antibody [EPR23228-40] (ab245228) at 1/1000 dilution

    Lane 1 : Mouse P0 cerebellum tissue lysate
    Lane 2 : Mouse thymus tissue lysate
    Lane 3 : Rat thymus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23747308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat skin (PMID: 21514423, 21514423).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Flow Cytometry - Anti-EGR2 antibody [EPR23228-40] (ab245228)

    Flow cytometric analysis of 2% paraformaldehyde fixed, True-Nuclear buffer-permeabilized Mouse splenocyte (treated with 5ug/ml anti-mouse CD3 and 2ug/ml anti-mouse CD28 for 16h) cells labelling EGR2 with ab245228 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD4 conjugated to APC-Cy7 . Then fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG (Left) or ab245228 (Right).

  • Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Western blot - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Anti-EGR2 antibody [EPR23228-40] (ab245228) at 1/1000 dilution + Rat brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Expopsure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23747308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the rat cerebrum.The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR2 antibody [EPR23228-40] (ab245228)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling EGR2 with ab245228 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). IHC results showed nuclear staining on the mouse cerebrum (PMID: 30122531).The section was incubated with ab245228 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Anti-EGR2 antibody [EPR23228-40] (ab245228)
    Anti-EGR2 antibody [EPR23228-40] (ab245228)

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