Anti-ZNF217 antibody (ab48133)
Key features and details
- Rabbit polyclonal to ZNF217
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ZNF217 antibody
See all ZNF217 primary antibodies -
Description
Rabbit polyclonal to ZNF217This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
-
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human ZNF217.
Read Abcam's proprietary immunogen policy (Peptide available as ab48177.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MousePigApplication Abreviews Notes WB (1) Notes Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab48177).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
-
Function
Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7764 Human
- Entrez Gene: 228913 Mouse
- Omim: 602967 Human
- SwissProt: O75362 Human
- Unigene: 155040 Human
- Unigene: 711564 Human
-
Alternative names
- ZABC 1 antibody
- ZABC1 antibody
- Zinc finger protein 217 antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Western blot - Anti-ZNF217 antibody (ab48133)Anti-ZNF217 antibody (ab48133) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate at 8 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (5)
ab48133 has been referenced in 5 publications.
- Song T et al. Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation. Nucleic Acids Res N/A:N/A (2019). PubMed: 31037292
- Serandour AA et al. TRPS1 regulates oestrogen receptor binding and histone acetylation at enhancers. Oncogene 37:5281-5291 (2018). PubMed: 29895970
- Baran-Gale J et al. An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells. RNA 22:1592-603 (2016). WB ; Human . PubMed: 27539783
- Cohen PA et al. The dark side of ZNF217, a key regulator of tumorigenesis with powerful biomarker value. Oncotarget 6:41566-81 (2015). PubMed: 26431164
- Nguyen NT et al. A functional interplay between ZNF217 and estrogen receptor alpha exists in luminal breast cancers. Mol Oncol 8:1441-57 (2014). PubMed: 24973012
Images
-
Western blot - Anti-ZNF217 antibody (ab48133)Anti-ZNF217 antibody (ab48133) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate at 8 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
