Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)
![Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.com/ps/products/81/ab81440/Images/ab81440-96627-anti-egfr-phospho-y992-antibody-em-12-western-blot.jpg "Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)")
Key features and details
- Mouse monoclonal [EM-12] to EGFR (phospho Y992)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-EGFR (phospho Y992) antibody [EM-12]
See all EGFR primary antibodies -
Description
Mouse monoclonal [EM-12] to EGFR (phospho Y992) -
Host species
Mouse -
Specificity
ab81440 recognises EGFR phosphorylated on tyrosine 992. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phospho-peptide covering sequence around tyrosine 992 of Human EGFR.
-
Positive control
- EGF-stimulated A431. A431, CALU-3, Jurkat cell lines.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity: > 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
EM-12 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)All lanes : Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440) at 1 µg/ml
Lane 1 : EGF-treated A431 cell line
Lane 2 : EGF-treated CALU-3 cell line
Lane 3 : EGF-treated MCF-7 cell line
Lane 4 : EGF-treated Jurkat cell line
Lane 5 : EGF-treated Ramos cell line
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
-
![Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.com/ps/products/81/ab81440/Images/ab81440-96626-anti-egfr-phospho-y992-antibody-em-12-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with ab81440 at 1µg/ml and goat anti-mouse-HRP. -
![Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)](https://www.abcam.com/ps/products/81/ab81440/Images/ab81440-96625-anti-egfr-phospho-y992-antibody-em-12-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
