Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)
![Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)](https://www.abcam.com/ps/products/68/ab68470/Images/ab68470-82762-anti-egfr-phospho-y1086-antibody-ep2258ihcy-western-blot.jpg "Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-EGFR (phospho Y1086) antibody [EP2258IHCY]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086) -
Host species
Rabbit -
Specificity
Recognises EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1 (corresponding to Y1110 from the precursor form P00533-1/p170) -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
corresponding to EGFR (phospho Y1086).
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Positive control
- A431 cell lysate, A431 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2258IHCY -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)All lanes : Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470) at 1/5000 dilution
Lane 1 : A431 cell lysate, untreated.
Lane 2 : A431 cell lysate, treated with EGF.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugatecd Goat anti-rabbit at 1/2000 dilution
Predicted band size: 134 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)](https://www.abcam.com/ps/products/68/ab68470/Images/ab68470-82761-anti-egfr-phospho-y1086-antibody-ep2258ihcy-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)A431 cells stained with ab68470 at 1/100 dilution. -
![Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)](https://www.abcam.com/ps/products/68/ab68470/Images/ab68470-3-benefits-of-recombinant-antibodies.png)
Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)
