Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086)
Suitable for: ICC/IF, WB
Reacts with: Human

Product name

Anti-EGFR (phospho Y1086) antibody [EP2258IHCY]
See all EGFR primary antibodies
Description

Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086)
Host species

Rabbit
Specificity

Recognises EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1 (corresponding to Y1110 from the precursor form P00533-1/p170)
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

corresponding to EGFR (phospho Y1086).
Positive control
- A431 cell lysate, A431 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP2258IHCY
Isotype

IgG
Research areas

Western blot - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)

All lanes : Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470) at 1/5000 dilution

Lane 1 : A431 cell lysate, untreated.
Lane 2 : A431 cell lysate, treated with EGF.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugatecd Goat anti-rabbit at 1/2000 dilution

Predicted band size: 134 kDa
Observed band size: 200 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)

A431 cells stained with ab68470 at 1/100 dilution.
Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com