Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9471] to eEF1A1/EF-Tu
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-eEF1A1/EF-Tu antibody [EPR9471]
See all eEF1A1/EF-Tu primary antibodies
Description

Rabbit monoclonal [EPR9471] to eEF1A1/EF-Tu
Host species

Rabbit
Specificity

The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human eEF1A1/EF-Tu aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: P68104
Positive control
- WB: HeLa, MCF7, 293T and Neuro-2a cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9471
Isotype

IgG
Research areas

Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

All lanes : Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455) at 1/40000 dilution (purified)

Lane 1 : Rat kidney lysate
Lane 2 : Rat spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 50 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling eEF1A1/EF-Tu with purified ab157455 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling eEF1A1/EF-Tu with purified ab157455 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Flow Cytometry - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling eEF1A1/EF-Tu with purified ab157455 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455) at 1/40000 dilution (purified) + Mouse kidney lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 50 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

All lanes : Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455) at 1/50000 dilution (purified)

Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20 µg (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 50 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

All lanes : Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Neuro-2a cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa
Immunoprecipitation - Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

ab157455 (purified) at 1/30 immunoprecipitating eEF1A1/EF-Tu in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM/TBST.
Anti-eEF1A1/EF-Tu antibody [EPR9471] (ab157455)

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