Anti-E. coli LPS antibody [2D7/1] (ab35654)
![Anti-E. coli LPS antibody [2D7/1] (ab35654)](https://www.abcam.com/ps/products/35/ab35654/Images/ab35654-48358-anti-e-coli-lps-antibody-2d7-1-immunofluorescence.jpg "Anti-E. coli LPS antibody [2D7/1] (ab35654)")
Key features and details
- Mouse monoclonal [2D7/1] to E. coli LPS
- Suitable for: ICC/IF, ELISA
- Reacts with: Escherichia coli
- Isotype: IgG2b
Overview
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Product name
Anti-E. coli LPS antibody [2D7/1] -
Description
Mouse monoclonal [2D7/1] to E. coli LPS -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Tissue, cells or virus corresponding to Escherichia coli E. coli LPS. Escherichia coli J5 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2D7/1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-E. coli LPS antibody [2D7/1] (ab35654) This image is courtesy of an anonymous Abreview
ab35654 staining E. coli LPS in mouse hepatocytes (red). Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in SFX Kit blocking agent for 30 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 555 goat anti-mouse, diluted 1/100. Cells were stimulated for 90 minutes with E. coli LPS and then probed with ab35654. The picture was taken on a Leica confocal microscope that pseudo-colors the 555 secondary red. The blue staining was with To-Pro-III Iodide,again, pseudo-colored to be blue on confocal.