Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)

Key features and details

  • Mouse monoclonal [1E6] to Salmonella typhimurium LPS
  • Suitable for: WB, Dot blot, ELISA, ICC/IF, IHC-Fr
  • Reacts with: Salmonella typhimurium
  • Isotype: IgG1

Overview

  • Product name

    Anti-Salmonella typhimurium LPS antibody [1E6]

  • Description

    Mouse monoclonal [1E6] to Salmonella typhimurium LPS

  • Host species

    Mouse

  • Specificity

    This antibody is directed against lipopolisaccharides of Salmonella typhimurium

  • Tested applications

    Suitable for: WB, Dot blot, ELISA, ICC/IF, IHC-Frmore details

  • Species reactivity

    Reacts with: Salmonella typhimurium

  • Immunogen

    Lipopolysaccharides of Salmonella typhimurium

  • General notes

    This antibody is used for the detection of LPS of Salmonella typhimurium.

    This product was changed from ascites to tissue culture supernatant on 16th July 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)
    Immunohistochemistry (Frozen sections) - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) This image is courtesy of an anonymous Abreview
    Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.

    See Abreview

  • Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)
    Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) Image courtesy of Aaron White by Abreview.
    All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution

    Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
    Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
    Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
    Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344

    Lysates/proteins at 100000000 cells per lane.

    Secondary
    All lanes : AP conjugated goat anti-mouse polyclonal at 1/4000 dilution


    Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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