Mouse GRO gamma Matched Antibody Pair Kit (ab212070)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.6 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse GRO gamma Matched Antibody Pair Kit
See all GRO gamma kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
7.08 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GRO gamma Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse GRO gamma.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse GRO Gamma Capture Antibody 2 x 50µg 1 x 50µg Mouse GRO Gamma Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse GRO Gamma Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 3
- C-X-C motif chemokine ligand 3
- Chemokine (C X C motif) ligand 3
see all -
Database links
- Entrez Gene: 330122 Mouse
- SwissProt: Q6W5C0 Mouse
- Unigene: 244289 Mouse
Images
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Mouse GRO Gamma standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse GRO gamma Matched Antibody Pair Kit (ab212070)To learn more about the advantages of recombinant antibodies see here.
