Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Wednesday March 10, 2021

Anti-DTN-A antibody [EPR14112] (ab191395)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14112] to DTN-A
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-DTN-A antibody [EPR14112]
See all DTN-A primary antibodies
Description

Rabbit monoclonal [EPR14112] to DTN-A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, Human cerebellum and skeletal muscle lysats; A673 and U87-MG cell lysates; Human thyroid carcinoma tissue; U87-MG and A673 cells.
General notes
Previously labelled as Dystrobrevin alpha.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14112
Isotype

IgG
Research areas

Western blot - Anti-DTN-A antibody [EPR14112] (ab191395)

All lanes : Anti-DTN-A antibody [EPR14112] (ab191395) at 1/10000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : Human skeletal muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 84 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DTN-A antibody [EPR14112] (ab191395)

Immunofluorescent analysis of U87-MG cells (4% formaldehyde-fixed; 0.1% Triton X-100 permeabilized) labeling DTN-A with ab191395 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at 1/200 dilution. Counter-stained with DAPI (blue). Negative controls: Primary ab concentration (anti-DTNA) is 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.
Western blot - Anti-DTN-A antibody [EPR14112] (ab191395)

All lanes : Anti-DTN-A antibody [EPR14112] (ab191395) at 1/10000 dilution

Lane 1 : A673 cell lysate
Lane 2 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTN-A antibody [EPR14112] (ab191395)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling DTN-A with ab191395 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-DTN-A antibody [EPR14112] (ab191395)

Flow Cytometric analysis of A673 cells (paraformaldehyde-fixed, 2%) labeling DTN-A with ab191395 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-DTN-A antibody [EPR14112] (ab191395)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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