Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DTN-A antibody [EPR14112] (ab191395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14112] to DTN-A
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DTN-A antibody [EPR14112]
    See all DTN-A primary antibodies

  • Description

    Rabbit monoclonal [EPR14112] to DTN-A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Human cerebellum and skeletal muscle lysats; A673 and U87-MG cell lysates; Human thyroid carcinoma tissue; U87-MG and A673 cells.

  • General notes

     

     

    Previously labelled as Dystrobrevin alpha.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DTN-A antibody [EPR14112] (ab191395)
    Western blot - Anti-DTN-A antibody [EPR14112] (ab191395)
    All lanes : Anti-DTN-A antibody [EPR14112] (ab191395) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human cerebellum lysate
    Lane 3 : Human skeletal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 84 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DTN-A antibody [EPR14112] (ab191395)
    Immunocytochemistry/ Immunofluorescence - Anti-DTN-A antibody [EPR14112] (ab191395)

    Immunofluorescent analysis of U87-MG cells (4% formaldehyde-fixed; 0.1% Triton X-100 permeabilized) labeling DTN-A with ab191395 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at 1/200 dilution. Counter-stained with DAPI (blue). Negative controls: Primary ab concentration (anti-DTNA) is 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. 

  • Western blot - Anti-DTN-A antibody [EPR14112] (ab191395)
    Western blot - Anti-DTN-A antibody [EPR14112] (ab191395)
    All lanes : Anti-DTN-A antibody [EPR14112] (ab191395) at 1/10000 dilution

    Lane 1 : A673 cell lysate
    Lane 2 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTN-A antibody [EPR14112] (ab191395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DTN-A antibody [EPR14112] (ab191395)

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling DTN-A with ab191395 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-DTN-A antibody [EPR14112] (ab191395)
    Flow Cytometry - Anti-DTN-A antibody [EPR14112] (ab191395)

    Flow Cytometric analysis of A673 cells (paraformaldehyde-fixed, 2%) labeling DTN-A with ab191395 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-DTN-A antibody [EPR14112] (ab191395)
    Anti-DTN-A antibody [EPR14112] (ab191395)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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