Anti-Dopamine D2 Receptor antibody (ab85367)
Key features and details
- Rabbit polyclonal to Dopamine D2 Receptor
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dopamine D2 Receptor antibody
See all Dopamine D2 Receptor primary antibodies -
Description
Rabbit polyclonal to Dopamine D2 Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chimpanzee, Monkey
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Immunogen
Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97357) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human brain; Human skeletal muscle; Rat Substantia nigra; Rat Dopaminergic nucleus.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab85367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa). Target
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Function
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. -
Involvement in disease
Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1813 Human
- Entrez Gene: 13489 Mouse
- Entrez Gene: 24318 Rat
- Omim: 126450 Human
- SwissProt: P14416 Human
- SwissProt: P61168 Mouse
- SwissProt: P61169 Rat
- Unigene: 73893 Human
see all -
Alternative names
- D(2) dopamine receptor antibody
- D2 dopamine receptor antibody
- D2DR antibody
see all
Images
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Western blot - Anti-Dopamine D2 Receptor antibody (ab85367)All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
Western blot - Anti-Dopamine D2 Receptor antibody (ab85367)All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml
Lane 1 : Rat Substantia Nigra Lysate
Lane 2 : Dopaminergic nucleus Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa (possible glycosylated form)
Exposure time: 4 minutes
Datasheets and documents
References (14)
ab85367 has been referenced in 14 publications.
- Kupnicka P et al. Fluoride Affects Dopamine Metabolism and Causes Changes in the Expression of Dopamine Receptors (D1R and D2R) in Chosen Brain Structures of Morphine-Dependent Rats. Int J Mol Sci 21:N/A (2020). PubMed: 32235357
- Eom TY et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun 11:912 (2020). PubMed: 32060266
- Xu X et al. Inhibition of Methamphetamine Self-Administration and Reinstatement by Central Blockade of Angiotensin II Receptor in Rats. J Pharmacol Exp Ther 369:244-258 (2019). PubMed: 30867225
- Regan SL et al. Knockout of latrophilin-3 in Sprague-Dawley rats causes hyperactivity, hyper-reactivity, under-response to amphetamine, and disrupted dopamine markers. Neurobiol Dis 130:104494 (2019). PubMed: 31176715
- Lin CC et al. Subchronic administration of aripiprazole improves fear extinction retrieval of Pavlovian conditioning paradigm in rats experiencing psychological trauma. Behav Brain Res 362:181-187 (2019). PubMed: 30610908
Images
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Western blot - Anti-Dopamine D2 Receptor antibody (ab85367)All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
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Western blot - Anti-Dopamine D2 Receptor antibody (ab85367)All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml
Lane 1 : Rat Substantia Nigra Lysate
Lane 2 : Dopaminergic nucleus Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa (possible glycosylated form)
Exposure time: 4 minutes
