Human Dopamine beta Hydroxylase Antibody Pair - BSA and Azide free (ab253463)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Dopamine beta Hydroxylase Antibody Pair - BSA and Azide free
See all Dopamine beta Hydroxylase kits -
Assay type
ELISA set -
Range
78.125 pg/ml - 5000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Human Dopamine beta Hydroxylase. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277076), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Dopamine beta Hydroxylase Capture Antibody (unconjugated) 1 x 100µg Human Dopamine beta Hydroxylase Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Conversion of dopamine to noradrenaline. -
Pathway
Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1. -
Involvement in disease
Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. -
Sequence similarities
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
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Alternative names
- dbh
- DBM
- Dopamine beta hydroxylase
see all -
Database links
- Entrez Gene: 1621 Human
- Omim: 609312 Human
- SwissProt: P09172 Human
- Unigene: 591890 Human
Images
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Sandwich ELISA - Human Dopamine beta Hydroxylase Antibody Pair - BSA and Azide free (ab253463)To learn more about the advantages of recombinant antibodies see here.
