Anti-DOCK4 antibody (ab85723)
Key features and details
- Rabbit polyclonal to DOCK4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK4 antibody -
Description
Rabbit polyclonal to DOCK4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1725-1775 of Human DOCK4 NP_055520.3
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Positive control
- HeLa whole cell lysate (ab150035), 293T cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DOCK4 with ab85723 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-DOCK4 antibody (ab85723) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 225 kDa
Observed band size: 225 kDa
Exposure time: 30 seconds
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Detection of Human DOCK4 by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded)using ab85723 at 3ug/mg lysate. Subsequent Western blot detection was performed using 0.4 ug/ml ab85723. Detection: Chemiluminescence with exposure times of 30 seconds.