Anti-Serum Response Factor SRF antibody (ab53147)
Key features and details
- Rabbit polyclonal to Serum Response Factor SRF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Serum Response Factor SRF antibody
See all Serum Response Factor SRF primary antibodies -
Description
Rabbit polyclonal to Serum Response Factor SRF -
Host species
Rabbit -
Specificity
ab53147 detects endogenous levels of total Serum Response Factor SRF protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 99 (S-L-S-E-M).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53147 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Serum Response Factor SRF antibody (ab53147)All lanes : Anti-Serum Response Factor SRF antibody (ab53147) at 1/300 dilution
Lane 1 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with no peptide
Lane 2 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with peptide
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody (ab53147)Immunohistochemical analysis of Serum Response Factor SRF in paraffin embedded human breast carcinoma tissue using ab53147 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
