Anti-Serum Response Factor SRF antibody (ab245462)
Key features and details
- Rabbit polyclonal to Serum Response Factor SRF
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Serum Response Factor SRF antibody
See all Serum Response Factor SRF primary antibodies -
Description
Rabbit polyclonal to Serum Response Factor SRF -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Serum Response Factor SRF aa 25-75. The exact sequence is proprietary. NP_003122.1
Database link: P11831 -
Positive control
- WB: HeLa, jurkat and HEK-293T whole cell lysate. IP: HeLa cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245462 was affinity purified using an epitope specific to Serum Response Factor SRF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody (ab245462)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Serum Response Factor SRF using ab245462 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-Serum Response Factor SRF antibody (ab245462)Serum Response Factor SRF was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab245462 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab245462 at 1 µg/ml.
Lane 1: ab245462 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western blot - Anti-Serum Response Factor SRF antibody (ab245462)All lanes : Anti-Serum Response Factor SRF antibody (ab245462) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutes
